Volgograd state medical university, Volgograd, e-mail: [email protected]
The most important link in structure of medico-biological experiment is laboratory animals. Requirements of scientists to quality of laboratory animals, to their standardization on a genotype, maintenance and feeding conditions, testability on micro-flora and parasitic organisms now have essentially increased. Laboratory animals are subject to various diseases. For example, very common situations are exogenous parasitoses, such as trichodectoses and sarcoptoses. Frequently sarcoptoses and trichodectoses meet in association. Despite the many new pharmaceuticals for treatment of this exoparasitoses in the Russian veterinary market, there are no accurate schemes of treatment and dosages for rodents. Summarising the above-stated and considering a wide circulation of sarcoptoses and trichodectoses, it was found expedient to investigate a spectrum of insecticid and acaricid activity of preparations accessible in veterinary drugstores of Volgograd (Russia). It is revealed, that the most presented preparations are «NeoStomosan», «Celandin Sprey», «Leopard», «Frontline Sprey» and «Zoopowder Puldis». Three from these («Leopard», «NeoStomosan», «Zoopowder Puldis») have been chosen for estimation of acaricid and insecticid effects. All preparations are recommended for struggle with parasitic invasions of cats and dogs whereas an exact dosage for small rodents is not revealed.
The experiments were carried out on 120 outbreed sexually mature male rats (190-210 g), according to the international norms and rules of work with vertebrate animals (Strasbourg, 1999). It was confirmed clinically and morphologically that all animals were parasitized with the following: sarcoptos bodies, ears, superciliary arches and expressed diffuse trichodectoses. Animals have been divided into 4 equivalent groups. Animals of the first group were exposed by the «Leopard» spray, the second group – by «Leopard» drops, the third group was processed by «Puldis», and the fourth group – by «NeoStomosan». The degree of parasitic invasion was estimated every day on an original scale by «Estimations of an external condition of laboratory animals» within 6 days.
Our results indicate that the activity degree of the investigated preparations can be distributed as: «Leopard»> «Puldis»> «NeoStomosan», and by efficiency (the speed of full clearing from parasites) – as: «Puldis»> «Leopard»> «NeoStomosan».